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No mutants had significant numbers of asci with more than four spores.
Fully developed ascospores within intact asci could be identified by their red pigmentation.
Hymenium - the spore-bearing layer of a fruiting body, containing asci , spores and paraphyses.
Spores were liberated from asci , and vegetative cells were killed, by treatment with glusulase and ethanol.
When mature, a perithecium contains several hundred asci that are ejected by turgor pressure.
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